diff --git a/Marlin/Configuration.h b/Marlin/Configuration.h
index 2a64000449..a3c762a72e 100644
--- a/Marlin/Configuration.h
+++ b/Marlin/Configuration.h
@@ -2674,9 +2674,6 @@
 // Touch-screen LCD for Malyan M200/M300 printers
 //
 //#define MALYAN_LCD
-#if ENABLED(MALYAN_LCD)
-  #define LCD_SERIAL_PORT 1  // Default is 1 for Malyan M200
-#endif
 
 //
 // Touch UI for FTDI EVE (FT800/FT810) displays
@@ -2690,7 +2687,6 @@
 //#define ANYCUBIC_LCD_I3MEGA
 //#define ANYCUBIC_LCD_CHIRON
 #if EITHER(ANYCUBIC_LCD_I3MEGA, ANYCUBIC_LCD_CHIRON)
-  #define LCD_SERIAL_PORT 3  // Default is 3 for Anycubic
   //#define ANYCUBIC_LCD_DEBUG
 #endif
 
@@ -2698,9 +2694,6 @@
 // 320x240 Nextion 2.8" serial TFT Resistive Touch Screen NX3224T028
 //
 //#define NEXTION_TFT
-#if ENABLED(NEXTION_TFT)
-  #define LCD_SERIAL_PORT 1  // Default is 1 for Nextion
-#endif
 
 //
 // Third-party or vendor-customized controller interfaces.
diff --git a/Marlin/Configuration_adv.h b/Marlin/Configuration_adv.h
index 4fdec050b5..ab0b7cdf20 100644
--- a/Marlin/Configuration_adv.h
+++ b/Marlin/Configuration_adv.h
@@ -1748,7 +1748,6 @@
 // Additional options for DGUS / DWIN displays
 //
 #if HAS_DGUS_LCD
-  #define LCD_SERIAL_PORT 3
   #define LCD_BAUDRATE 115200
 
   #define DGUS_RX_BUFFER_SIZE 128
diff --git a/Marlin/src/inc/Conditionals_LCD.h b/Marlin/src/inc/Conditionals_LCD.h
index 45e48439f4..52c6e34f22 100644
--- a/Marlin/src/inc/Conditionals_LCD.h
+++ b/Marlin/src/inc/Conditionals_LCD.h
@@ -485,15 +485,6 @@
 // E3V2 extras
 #if HAS_DWIN_E3V2 || IS_DWIN_MARLINUI
   #define SERIAL_CATCHALL 0
-  #ifndef LCD_SERIAL_PORT
-    #if MB(BTT_SKR_MINI_E3_V1_0, BTT_SKR_MINI_E3_V1_2, BTT_SKR_MINI_E3_V2_0, BTT_SKR_MINI_E3_V3_0, BTT_SKR_E3_TURBO)
-      #define LCD_SERIAL_PORT 1
-    #elif MB(CREALITY_V24S1_301, CREALITY_V24S1_301F4, CREALITY_V423)
-      #define LCD_SERIAL_PORT 2 // Creality Ender3S1 board
-    #else
-      #define LCD_SERIAL_PORT 3 // Creality 4.x board
-    #endif
-  #endif
   #define HAS_LCD_BRIGHTNESS 1
   #define LCD_BRIGHTNESS_MAX 250
   #if ENABLED(DWIN_LCD_PROUI)
diff --git a/Marlin/src/inc/Conditionals_adv.h b/Marlin/src/inc/Conditionals_adv.h
index f82bd9b740..7a0c5dd934 100644
--- a/Marlin/src/inc/Conditionals_adv.h
+++ b/Marlin/src/inc/Conditionals_adv.h
@@ -1012,6 +1012,24 @@
   #define HAS_USER_ITEM(N) 0
 #endif
 
+/**
+ * LCD_SERIAL_PORT must be defined ahead of HAL.h
+ */
+#ifndef LCD_SERIAL_PORT
+  #if HAS_DWIN_E3V2 || IS_DWIN_MARLINUI
+    #if MB(BTT_SKR_MINI_E3_V1_0, BTT_SKR_MINI_E3_V1_2, BTT_SKR_MINI_E3_V2_0, BTT_SKR_MINI_E3_V3_0, BTT_SKR_E3_TURBO)
+      #define LCD_SERIAL_PORT 1
+    #elif MB(CREALITY_V24S1_301, CREALITY_V24S1_301F4, CREALITY_V423)
+      #define LCD_SERIAL_PORT 2 // Creality Ender3S1 board
+    #else
+      #define LCD_SERIAL_PORT 3 // Creality 4.x board
+    #endif
+  #endif
+  #ifdef LCD_SERIAL_PORT
+    #define AUTO_ASSIGNED_LCD_SERIAL 1
+  #endif
+#endif
+
 #if !HAS_MULTI_SERIAL
   #undef MEATPACK_ON_SERIAL_PORT_2
 #endif
diff --git a/Marlin/src/inc/SanityCheck.h b/Marlin/src/inc/SanityCheck.h
index 4b7317d96a..b4259bf53a 100644
--- a/Marlin/src/inc/SanityCheck.h
+++ b/Marlin/src/inc/SanityCheck.h
@@ -2941,6 +2941,8 @@ static_assert(Y_MAX_LENGTH >= Y_BED_SIZE, "Movement bounds (Y_MIN_POS, Y_MAX_POS
     #error "The ANYCUBIC LCD requires LCD_SERIAL_PORT to be defined."
   #elif ENABLED(MALYAN_LCD)
     #error "MALYAN_LCD requires LCD_SERIAL_PORT to be defined."
+  #elif ENABLED(NEXTION_LCD)
+    #error "NEXTION_LCD requires LCD_SERIAL_PORT to be defined."
   #endif
 #endif
 
diff --git a/Marlin/src/inc/Warnings.cpp b/Marlin/src/inc/Warnings.cpp
index 7b4371e700..ff38c47a37 100644
--- a/Marlin/src/inc/Warnings.cpp
+++ b/Marlin/src/inc/Warnings.cpp
@@ -67,6 +67,10 @@
 
 #ifndef NO_AUTO_ASSIGN_WARNING
 
+  #if AUTO_ASSIGNED_LCD_SERIAL
+    #warning "Note: Auto-assigned LCD_SERIAL_PORT. (Define NO_AUTO_ASSIGN_WARNING to suppress this warning.)"
+  #endif
+
   #if AUTO_ASSIGNED_X2_STEPPER
     #warning "Note: Auto-assigned X2 STEP/DIR/ENABLE_PINs to unused En_STEP/DIR/ENABLE_PINs. (Define NO_AUTO_ASSIGN_WARNING to suppress this warning.)"
   #endif