diff --git a/Marlin/src/HAL/HAL_ESP32/i2s.cpp b/Marlin/src/HAL/HAL_ESP32/i2s.cpp
index c503a24bf8..42e65eacd7 100644
--- a/Marlin/src/HAL/HAL_ESP32/i2s.cpp
+++ b/Marlin/src/HAL/HAL_ESP32/i2s.cpp
@@ -303,9 +303,9 @@ int i2s_init() {
   xTaskCreate(stepperTask, "StepperTask", 10000, NULL, 1, NULL);
 
   // Route the i2s pins to the appropriate GPIO
-  gpio_matrix_out_check(22, I2S0O_DATA_OUT23_IDX, 0, 0);
-  gpio_matrix_out_check(25, I2S0O_WS_OUT_IDX, 0, 0);
-  gpio_matrix_out_check(26, I2S0O_BCK_OUT_IDX, 0, 0);
+  gpio_matrix_out_check(I2S_DATA, I2S0O_DATA_OUT23_IDX, 0, 0);
+  gpio_matrix_out_check(I2S_BCK, I2S0O_BCK_OUT_IDX, 0, 0);
+  gpio_matrix_out_check(I2S_WS, I2S0O_WS_OUT_IDX, 0, 0);
 
   // Start the I2S peripheral
   return i2s_start(I2S_NUM_0);
diff --git a/Marlin/src/HAL/HAL_ESP32/i2s.h b/Marlin/src/HAL/HAL_ESP32/i2s.h
index 32999f712f..337d91b203 100644
--- a/Marlin/src/HAL/HAL_ESP32/i2s.h
+++ b/Marlin/src/HAL/HAL_ESP32/i2s.h
@@ -29,3 +29,9 @@ int i2s_init();
 void i2s_write(uint8_t pin, uint8_t val);
 
 void i2s_push_sample();
+
+// pin definitions
+
+#define I2S_WS 25
+#define I2S_BCK 26
+#define I2S_DATA 27